It is now possible to measure the biogenic amines histamine, tryptamine, putrescine, cadaverine, spermidine, spermine, and tyramine in an easy, accurate, and selective manner. Dansyl chloride was used to purify the analytes, and high-performance liquid chromatography with detection of fluorescence was utilized for separating them. This is a useful method for identifying and measuring biogenic amines in fish samples in a selective manner. The C18 (250 mm × 4.6 mm × 5 μm) column was used for the separation by chromatography. A mobile phase comprising acetonitrile and phosphate buffer (pH=4.0) (60:40, v/v) was used at an average flow rate of 0.9 mL/min. The technique relied on utilizing fluorescence detection to determine the derivative (λex=230 nm, λem= 350 nm) The suggested method's linearity was examined between 0.01 and 50 μg/mL (r2=0.9996). In compliance with ICH criteria, the method was verified with respect to accuracy, precision, repeatability, specifity, robustness, and detection and quantification limitations. The suggested technique has been effective in tracking the production of biogenic amines in specimens of fish that can be purchased frozen in marketplaces. It was discovered that the recommended approach worked well for routinely analyzing fish sample biogenic amine analyses.
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