This study reports the layered double hydroxides’ modified wheat straw biochar (WsBio-LDH composite) composite as an effective adsorbent for the removal of copper ions (Cu²⁺) from aqueous solution. The WsBio-LDH composite adsorbent was analyzed using central composite design and response surface methodology (RSM). RSM optimized parameters for Cu²⁺ removal using the WsBio-LDH composite were found to be 87.06 min for the contact time, 0.491 g for the adsorbent dose, 5.93 for the solution pH, and 12.75 mg/L for the initial Cu²⁺ concentration. A significant correlation between predicted and experimental values was observed with 99 % removal exhibiting a deviation of less than 1% that validates the model's accuracy. All individual batch variables significantly (p<0.0001) influenced the Cu²⁺ removal efficiency using the WsBio-LDH composite. Both the contact time and initial Cu²⁺ concentrations displayed negative effects while the solution pH and Cu²⁺ concentrations exerted a positive interactive effect (p <0.0049) on Cu²⁺ removal efficiency using the WsBio-LDH composite. The Langmuir model accurately represented a monolayer adsorption of Cu²⁺ onto the surface of the WsBio-LDH composite. The pseudo-second order kinetic model provided the best fit for the adsorption kinetics of the WsBio-LDH composite. FTIR spectra revealed various functional groups on the surface of the WsBio-LDH composite before and after Cu²⁺ adsorption. The SEM images indicated a rough surface with two distinct phases of raw biochar and micro-sized Ca/Al/Mg-LDH particles, and roughness enhances further after the Cu²⁺ adsorption. EDX analysis further confirms the adsorption of Cu²⁺. These results underscore the efficacy of the WsBio-LDH composite as a promising nano-adsorbent for wastewater treatment applications.