Skip to main content

Application of free and immobilized laccase for removal and detoxification of fluoroquinolones from aqueous solution

  • Authors (legacy)
    Corresponding: Amir Hossein Mahvi, Mohammad Ali Faramarzi
    Co-authors: Ashrafi S.D.
    Nasseri S.
    Alimohammadi M.
    Mahvi A.H.
    Faramarzi M.A.
    Download PDF
  • gnest_02973_published.pdf
  • Paper ID
    gnest_02973
  • Paper status
    Published
  • Date paper submitted
  • Date paper accepted
  • Date paper online
DOI
https://doi.org/10.30955/gnj.002973
Graphical abstract
Abstract

Laccase from Trametes versicolor was immobilized by covalent bonds formation on CPC silica carriers. Elimination of two floroqinolone (FQ); enrofloxacine (ENR) and flumequine (FLU) using laccase in both free and immobilized form in the absence and presence of 1-hydroxybenzotriazole (HBT) and 4-Hydroxybenzoic acid (HBA) as mediators was investigated. Temperature, pH and storage stability of immobilized laccase was significantly improved compare to free laccase. In the absence of a laccase mediator, the initial concentrations of 50 mg L–1 of ENR and FLU decreased by 19 % and 28 %, respectively, after 6 h treatment using the immobilized laccase, while, the removal percentages were increased to 98 % and 96 %, respectively, when the immobilized laccase was used in presence of HBT. Whereas, the removal percentages of ENR and FLU were increased to 97 % and 88 %, respectively, when the immobilized laccase was used in presence of HBA. After twenty runs of the enzymatic elimination (laccase-HBT system) of ENR and FLU, the immobilized laccase exhibited the relative removal of 17.63 % and 15.62 %, respectively. The results of microtoxicity test (growth inhabitation percentage of six bacterial strains) showed a significant decrease in toxicity of the laccase-treated ENR and FLU solution.

Keywords
Enrofloxacine Flumequine Immobilized enzyme Laccase Antibiotic removal.
Cite this article
Faramarzi, M.A. et al. (2020) “Application of free and immobilized laccase for removal and detoxification of fluoroquinolones from aqueous solution ”, Global NEST Journal, 22(2). Available at: https://doi.org/10.30955/gnj.002973.
PDF file
  • gnest_02973_published.pdf