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Optimisation of enzymatic fermented glucose production of wild cocoyam starch using response surface methodology

Paper Topic: 
Biodiversity conservation
 
Volume: 
 
 

Pages :
351 - 361

Corresponing Author: 
Charles Odilichukwu R. Okpala
 
Authors: 
Uchegbu NN Ude CN Okoyeuzu CF Nwadi OMM Rasaq WA Ogbonna CU Hossaini SM Okpala COR
Paper ID: 
gnest_03927
Paper Status: 
Published
Date Paper Accepted: 
10-04-2022
Paper online: 
23/5/2022
Visual abstract: 
Abstract: 

​The enzymatic fermentation of starch from non-edible sources using Aspergillus niger could help supplement the increasing glucose demand within the Nigeria food supply chain. Also, response surface methodology could help optimise the glucose production of starch hydrolysed from cocoyam tubers. In this work, we evaluated and optimized the enzymatic-fermented glucose production of wild cocoyam starch using response surface methodology. Wild cocoyam starch was hydrolyzed using A. niger isolated from the soil. The optimization process involved temperature, time, pH, and enzyme dosage, alongside the kinetics and thermodynamics of enzymatic hydrolysis. Optimum conditions for glucose yield of 95 % by enzyme hydrolysis included: temperature = 35 °C; time = 5 days; pH = 5.5; and enzyme dosage =0.16 g/mL, with wild cocoyam showing promising as substrate. The F-value of quadratic model terms appeared statistically significant (F= 65.42, p<0.0001). Fourier Transform Infra-Red Spectrometer (FTIR) and Gas Chromatography-Mass Spectrophotometer (GC-MS) analyses confirmed characteristic bands of glucose as industrially promising with 60% purity. Further, the enzymatic hydrolysis adhered to Michaelis-Menten kinetic model with maximum reaction rate of 82.6 ml/day at 35 °C.

Keywords: 
Amylase enzyme, Aspergillus niger, Michaelis-Menten kinetic model, Response surface methodology, Wild cocoyam