Slaughterhouse wastewaters are characterized by a high organic content, mainly composed of proteins and fats. Therefore, these wastewaters should be treated efficiently prior to discharge into receiving bodies to avoid severe environmental pollution. This work aimed to characterize slaughterhouse wastewater generated in one province of Iran (Khuzestan), evaluating various suitability of biological treatment, assessing wastewater treatment plants performance and feasibility of wastewater reuse. Composite samples were collected from input and output of wastewater treatment plant during 6 months (spring and summer) and were analyzed for TSS, turbidity, temperature, conductivity, pH, COD, BOD5, fat, total coliform and fecal coliform. Data analysis was done using Excel and SPSS software. The results showed that different quantities of wastewater were generated in any slaughterhouses of Ahvaz (120-600 l/d.sheep), Dezful (110-550 l/d.sheep), and Shushtar (139-694 l/d.sheep). Khuzestan slaughterhouses wastewater is classified by pollution severity as strong wastewater. The BOD5/COD ranges from 0.3 to 0.5, which indicates applicability of biological treatment. Wastewater treatment plant of Ahvaz with anaerobic stabilization ponds and extended aeration activated sludge process has the highest removal efficiencies of pollutants. The result also indicated reuse of slaughterhouses effluent was not acceptable due to not comply with the standards of Iran. Finally, if safe use or disposal of these effluents is desired, blood capture from raw wastewater for reducing the amounts of organic loading must be implemented. Also, use of an appropriate treatment plant is noted.