Focusing on Eco-Friendly Biosorption Method: Removal of reactive yellow-145 by natural Ureolytic Mixed Culture

In this study, removal of reactive yellow 145 (RY 145) dyestuff by dry biomass of Ureolytic mixed microorganism culture (UMC), existing in domestic wastewater and various industrial wastewater, was studied. Optimum conditions of adsorption efficiency were tested. For this purpose, studies were conducted on the effect of initial dye concentration (30-50-75-100-150 mg/L), UMC amount (0.2-0.5 -1.0-2.0 g), temperature (20-35-50 °C), pH (2-3-5-7-9) and contact time (1-5-10-20-30-60-120 min) on the efficiency of color removal. As a result of these studies, optimum conditions were revealed with 150 mg/L initial RY 145 concentration, 0.2 g dry UMC amount, 150 rpm agitation speed, 20 0C temperature, pH 2 and 30. minute contact time. After spectrophotometric calculations, according to Langmuir isotherm, it was found that 610.9 mg/g and the removal efficiency of tests was calculated as 84.5% under optimum conditions. It is seen that the process in which physisorption is at the forefront and single-layer adsorption occurs fits the Langmuir and pseudo-second order models. Thermodynamic data showed that the process occurred spontaneously and endothermically. The results support that UMC is both a promising and alternative environmentally friendly biosorbent for RY 145 removal.

Keywords: Biosorption, Biosorbent, Dyestuff, Ureolytic Microorganism, Reactive Yellow 145