In the present research packed bed column with activated carbon which is derived from the natural available dates seed used for exclusion of perchlorate from aqueous sample. The effect of several variables on adsorption performance has been studied, including inlet ion concentration, flow rate, bed height, and temperature.The adsorbent was prepared in two different temperature 400℃ and 800℃ and the depth of column was 5cm and 8cm. The findings showed that the activated column at 400℃ in the depth of 5cm was saturated at 320 min and for 8cm column it is saturated at 300 min. Likewise for 800℃ columns in the depth of 5cm the treatment efficiency attained at 220 min and for 8cm column it was 180 min. It is showed that the optimal flow rate and bed height increase adsorption effectiveness. Gas chromatography (GC) was used to determine the perchlorate concentration in liquid sample after treatment process. The Thomas and Thomson mathematical models were utilized to determine the column's adsorption efficacy, with the maximum R2value of 0.985 for 800°C in an 8 cm column with a removal efficiency of 98.1%. The surface morphology was examined with SEM, FTIR and XRD which proves that the pore changes in the morphology of the adsorbent created homogeneous to heterogeneous formation structure. This comprehensive study shown that activated carbon generated from dates seed is a successful adsorbent for the removal of perchlorate at optimal temperature and bed depth