This research examines the scope for reducing the air pollution caused by the transportation sector through the infusion of biofuel and antioxidants ensuring the partial replacement of conventional fuel. A four-stroke, direct-injection, multi-cylinder and constant-speed diesel engine was employed for the experimental analysis. The ternary fuel blend with a composition of 70% diesel fuel, 20% rubber seed oil and 10% of pentanol and sea weed extract in different ratios were blended to form B20, B20A1, B20A2 and B20A3. The experimental results showed smoke and carbon monoxide reduced by 4.71%, unburned hydrocarbons by 2.2 %. The NOx emission which was slightly higher compared to diesel but not higher than B20 for the blend B20A2 by 5%. The brake thermal efficiency was increased by 3.71% when compared to diesel fuel. Consequently, the B20A2 (5% seaweed and 5% pentanol additive with 70% diesel and 20% of rubber seed methyl ester oil) was found to be an environmentally biodegradable gasoline with the best emission properties. The study findings are quite promising for future which seeks towards zero emission with optimum quantity of biofuel to replace diesel.