The performance of a Sequential Batch Biofilm Reactor (SBBR) for the treatment of grey water was evaluated using two different bio-carries under different aeration duration. Two biofilm carriers such as fujino spiral and loofah sponge was used for biofilm formation in the reactor. The study demonstrated that the SBBR system, when operated with both biofilm carriers, effectively removed contaminants from grey water. The aeration time of 45 minutes demonstrated the highest removal efficiencies for organic matter, and suspended solids for the both biofilm carrier i.e., fujino spiral and loofah sponge. Comparing the two biofilm carriers, the Fujino spiral showed strong biofilm attachment and demonstrated excellent performance in pollutant removal. The removal efficiencies of COD, BOD, TS, TDS was found out to be 94%, 93%, 72%, 73% respectively. On the other hand, the loofah sponge exhibited slightly lower performance compared to the Fujino spiral. However, it still proved to be an effective biofilm carrier, providing a suitable substrate for biofilm development and contributing to grey water treatment. The removal efficiencies of COD, BOD, TS, TDS was found out to be 92%, 93%, 65%, 63% respectively.