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Thematic area
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Issue
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Pages1-8
- gnest_05495_published.pdf
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Paper IDgnest_05495
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Paper statusPublished
![](/sites/default/files/submission_images/gnest_05495/20240113184410/gnest_05495-visual_abstract.png)
The biological synthesis of nanomaterials is drawing immense interest because of their non-hazardous nature and enormous antimicrobial application. In the present study, we explored Polygonatum geminiflorum Decne for phytochemical profiling and biosynthesis of silver nanoparticles to control soft rot/blackleg and bacterial wilt pathogens of potato through in vitro experiment. Phytochemical screening indicated the presence of important secondary chemicals including tannins, glycosides, flavonoids and terpenoids, while, gas chromatography-mass spectrophotometry (GC-MS) study of leaf extract showed the presence of 30 phytochemicals, the most prominent among which included ç-Sitosterol and n-Hexadecanoic acid. The GC–MS qualitative analysis also supported the presence of bioactive compounds responsible for metal reduction processes and synthesized nanoparticles stabilization. In vitro study showed that concentration of 100µg/mL of AgNPs and AgNPs-PE efficiently control both Erwinia carotovora and Ralstonia solanacearum. The outcomes have provided an improved protocol to use prepared AgNPs against the tested pathogens without health hazards.