This study examines the antidiabetic, photoprotective, and antibacterial properties of Asphodelus microcarpus (Liliaceae) essential oil (EO) collected from Constantine, Eastern Algeria. The EO was obtained by hydro-distillation and the composition was determined by Gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). Fifty-one (51) compounds were identified in the EO; the major components are α-phellandrene (15.18%), α-pinene (14.27%), Sabinene (13.81%), β-Ocimene (Z) (12.69%), and δ-3- carène (9.20%). Based on the findings of the biological assays, the EO appeared to have dose-dependent anti-diabetic activity in the α-amylase test, with an IC50 value of 412.73±5.83µg/ml. Additionally, according to the Recommendation of the European Commission (2006), this oil displayed significant activity with a sun protection factor (SPF) of 31.89±0.10. Furthermore, the EO was tested for its antibacterial activity against five bacteria strains, and its antifungal activity against Aspergillus niger using the agar-well diffusion method. The studied EO showed only marginal activity against the tested microorganisms and was inactive against Pseudomonas aeruginosa. However, A.microcarpus EO showed notable antifungal activity against Aspergillus niger with a minimum concentration of 25µg/mL. It is important to note that this is the first report on the α-amylase and SPF effects of Asphodelus microcarpus EO.