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Application of free and immobilized laccase for removal and detoxification of fluoroquinolones from aqueous solution View Crossref metadata(active tab) <div id="edit-xml" class="form-item form-type-item"> <?xml version="1.0"?> <doi_batch version="4.4.1" xmlns="http://www.crossref.org/schema/4.4.1" xmlns:jats="http://www.ncbi.nlm.nih.gov/JATS1" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.crossref.org/schema/4.4.1 http://www.crossref.org/schemas/crossref4.4.1.xsd"><head><doi_batch_id>000696</doi_batch_id><timestamp>1714094126</timestamp><depositor><depositor_name>University of the Aegean</depositor_name><email_address>uoa@university.com</email_address></depositor><registrant>Global NEST Journal</registrant></head><body><journal><journal_metadata><full_title>Global NEST Journal</full_title><full_title>Global NEST: the international Journal</full_title><issn media_type="print">1790-7632</issn><issn media_type="electronic">2241-777X</issn></journal_metadata><journal_issue><titles><title>Issue 2 </title></titles><publication_date><month>07</month><year>2024</year></publication_date><journal_volume><volume>22</volume></journal_volume><issue>2</issue></journal_issue><journal_article><titles><title>Application of free and immobilized laccase for removal and detoxification of fluoroquinolones from aqueous solution </title></titles><abstract xmlns="http://www.ncbi.nlm.nih.gov/JATS1"><p><p>Laccase from Trametes versicolor was immobilized by covalent bonds formation on CPC silica carriers. Elimination of two floroqinolone (FQ); enrofloxacine (ENR) and flumequine (FLU) using laccase in both free and immobilized form in the absence and presence of 1-hydroxybenzotriazole (HBT) and 4-Hydroxybenzoic acid (HBA) as mediators was investigated. Temperature, pH and storage stability of immobilized laccase was significantly improved compare to free laccase. In the absence of a laccase mediator, the initial concentrations of 50 mg L–1 of ENR and FLU decreased by 19 % and 28 %, respectively, after 6 h treatment using the immobilized laccase, while, the removal percentages were increased to 98 % and 96 %, respectively, when the immobilized laccase was used in presence of HBT. Whereas, the removal percentages of ENR and FLU were increased to 97 % and 88 %, respectively, when the immobilized laccase was used in presence of HBA. After twenty runs of the enzymatic elimination (laccase-HBT system) of ENR and FLU, the immobilized laccase exhibited the relative removal of 17.63 % and 15.62 %, respectively. The results of microtoxicity test (growth inhabitation percentage of six bacterial strains) showed a significant decrease in toxicity of the laccase-treated ENR and FLU solution.</p> </p></abstract><publication_date><month>07</month><day>06</day><year>2020</year></publication_date><pages><first_page>240</first_page><last_page>249</last_page></pages><publisher_item><identifier id_type="pii">gnest_02973</identifier></publisher_item><doi_data><doi>10.30955/gnj.002973</doi><timestamp>1714094126</timestamp><resource>https://journal.gnest.org/publication/gnest_02973</resource></doi_data></journal_article></journal></body></doi_batch> </div>
