<p>Removal of Al<sup>3+</sup> and Mn<sup>2+</sup> from solution by Trichoderma asperellum BHU216 immobilized on Irvingia gabonensis matrix in a batch system was investigated. Effect of biosorption conditions contact time, pH, bead size and spore load were studied. Equilibrium isotherms and re-usability of biosorbent were also determined. The result showed that optimum sorption of Mn<sup>2+ </sup>and Al<sup>3+</sup> by immobilized sorbent was achieved at 7 mm bead size and 1.6 g l<sup>-1</sup> spore load. Optimum pH were 5 and 6 while equilibrium was attained at 120 and 180 mins for Mn<sup>2+</sup> and Al<sup>3+</sup> sorption respectively. Immobilized biosorbent was used in more than 5 batch cycles with over 50% removal achieved in 3 and 5 cycles for Al<sup>3+</sup> and Mn<sup>2+</sup> sorption. Brunauer-Emmett-Teller (BET) isotherm had the best-fit than both Langmuir and Freundlich isotherms for both metals (R<sup>2</sup> =0.98 and 0.92 for Mn<sup>2+</sup> and Al<sup>3+</sup> respectively). Manganese sorption proceeded at both surface and multi-layer levels with high sorption rates 18.88 mg/L and 19.72 mg l<sup>-1</sup>, while aluminium sorption was feasible in a multi-layer process with low uptake (0.87 mg g<sup>-1</sup>). This study showed that immobilized T. asperellum BHU216 can effectively act as biosorbent for Mn(II) and Al(III) sorption from solution.</p>