Issue 1 [March]

A major solar salt works in Israel is located just north of the town of Eilat and close to the
airport. This solar salt works has been in operation since 1977 and produces about 170,000
tons a year. A three-year study was initiated to examine the temporal and salinity impacts on
the microbial community throughout this saltern. At the time this study was begun in the late
1990's, there were several paradigms about the microbial community:
1. there is greatly reduced diversity as the water reaches saturation;
2. because of the relative stability of the salt concentrations in the various pans, there is little
change in the microbial community; and,
3. halophilic Archaea are not found in ponds with less than 15% salt and halophilic Bacteria
would not be found in pans at saturation.
The first paradigm was developed based on a few studies using one medium and single
observations of various salterns. The second paradigm was an assumption that had not been
systematically tested, and the third paradigm, at least for the Bacteria, was shown in 1980 not
to be true with the discovery of the genus Halomonas.
We examined these paradigms by systematically studying on a seasonal basis the saltern at
Eilat, Israel, as well as the Cargill Solar Salt Plant in Newark, California. Both traditional and
molecular techniques have been used throughout these studies.
Several different approaches were taken: plate counts were made on several different media,
whole community carbon requirements were determined, molecular fingerprinting of the whole
microbial community using the amplicon length heterogeneity approach was performed to
determine the changes in the community composition over time, and partial characterization
of the isolated pure cultures were all performed. Samples were taken at least twice a year
corresponding to the cooler and hotter times of the year.
Based on this extensive study there were significant differences in the plate counts and in the
ratios of the various peaks found during the fingerprinting. It is safe to say now that the
microbial community in the waters of a solar saltern is variable and both domains can be
found throughout the saltworks.

We have carried out a geochemical study on the behaviour of certain trace elements
during the evaporative concentration of free brines (salinity from 41 to 400 ‰) of the solar
salt works of Sfax saline (S.E of Tunisia). The elements concerned by this survey are
Zinc, Cadmium, Manganese, Molybdenum, Lead, Copper, Aluminium, Iron and Barium.
Adequate analytical techniques, adapted to this environment of high salinity, have been
used to follow the evolution of the concentration of concerned elements in brines
subjected to the evaporation. The results obtained have been presented in function of
concentration factor calculated on the basis of the Lithium content in seawater (Coast of
Sfax) and those in the brines of salt works.
During the evaporation process, the concentration of trace elements in brines was
affected by the evaporation phenomenon for the same reason as the major elements.
Nevertheless, their concentrations remain very weak and don't reach their saturation
doorsteps. The participation of these elements in the mineral phases can take place only
by co-precipitation with saline paragenesis. It is the case of Zinc that can precipitate with
Sulphates Salts, the Cadmium, Barium and Copper with Carbonate and Gypsum, the
Manganese and the Molybdenum with Potassium Salts; others like Iron and Aluminium,
are characterized by a very complex behaviour and are subjected, therefore to effects
others than those of the evaporation and the co–precipitation. We can mention the
activity of the biological system and adsorption phenomena, in particular, on the organic
and mineral particles. They are frequent in ponds where brines are not yet very
concentrated; allowing an important biological productivity. Contents in these elements
are then variable and very dependent on the growth and the physiological state of the
organic matter. These micro-organisms use some metals in their metabolic activities, and notably those that act like vitamin factors. The analysis of sediment and algae sampled
from the first ponds of the saline, shows that they are capable as well to fix some
important quantities of trace elements. The liberation of these elements by deterioration
of organic matter, provoke the important fluctuations of their contents in free brines. In
basins where the biologic activity is very limited because of the increase of brines salinity,
the evolution of the concentration of trace elements translate the only effect of the
evaporation, counterbalanced by adsorption phenomenon and the co-precipitation with
the salts deposit.