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Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol.View Crossref metadata(active tab) <div id="edit-xml" class="form-item form-type-item"> <?xml version="1.0"?> <doi_batch version="4.4.1" xmlns="http://www.crossref.org/schema/4.4.1" xmlns:jats="http://www.ncbi.nlm.nih.gov/JATS1" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.crossref.org/schema/4.4.1 http://www.crossref.org/schemas/crossref4.4.1.xsd"><head><doi_batch_id>000689</doi_batch_id><timestamp>1713533312</timestamp><depositor><depositor_name>University of the Aegean</depositor_name><email_address>uoa@university.com</email_address></depositor><registrant>Global NEST Journal</registrant></head><body><journal><journal_metadata><full_title>Global NEST Journal</full_title><full_title>Global NEST: the international Journal</full_title><issn media_type="print">1790-7632</issn><issn media_type="electronic">2241-777X</issn></journal_metadata><journal_issue><titles><title>Issue 3</title></titles><publication_date><month>09</month><year>2024</year></publication_date><journal_volume><volume>17</volume></journal_volume><issue>3</issue></journal_issue><journal_article><titles><title>Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol.</title></titles><abstract xmlns="http://www.ncbi.nlm.nih.gov/JATS1"><p><div> <p>Petroleum products are used in different forms in auto-mechanic workshops every day. Spent motor oil disposed-off improperly contains potentially toxic substances which can seep into the water tables and contaminate ground water. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from three auto-mechanic workshops in Abeokuta, Ogun State. Total Heterotrophic Bacterial Counts ranged from 1.03 × 10<sup>6</sup> to 2.81 × 10<sup>6 </sup>CFU/g. Total Oil Degrading Bacterial Counts varied between 4.0 × 10<sup>5</sup> and 2.01 × 10<sup>6</sup> CFU/g while Surface Active Bacterial Counts were from 1.2 × 10<sup>4</sup> to 2.76 × 10<sup>5</sup> CFU/g. Twenty-four bacteria species capable of utilizing petroleum as a carbon source were isolated from various contaminated soils using enrichment technique. Isolated bacteria include: <em>Bacillus </em>spp., <em>Pseudomonas aeruginosa,</em> <em>Micrococcus </em>spp., <em>Proteus mirabilis</em>, <em>Proteus vulgaris </em>and<em> Enterobacter </em>spp. Redox indicator 2, 6-dichlorophenol indophenol (DCPIP) was used to screen for efficient hydrocarbon (Kerosene, Premium Motor Spirit, and Engine oil) degradation by bacteria. Degradation efficiency was measured by optical density at 600 nm. <em>Micrococcus </em>spp., <em>Proteus mirabilis</em>, and <em>Pseudomonas aeruginosa</em> were found to be the best isolates growing on majority of hydrocarbons due to their high utilization value when growing on the hydrocarbons tested.</p> </div> <p> </p> </p></abstract><publication_date><month>08</month><day>26</day><year>2015</year></publication_date><pages><first_page>565</first_page><last_page>573</last_page></pages><publisher_item><identifier id_type="pii">gnest_01514</identifier></publisher_item><doi_data><doi>10.30955/gnj.001514</doi><timestamp>1713533312</timestamp><resource>https://journal.gnest.org/publication/gnest_01514</resource></doi_data></journal_article></journal></body></doi_batch> </div>
