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Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

Paper Topic: 
General
 
Volume: 
 
Issue: 
 

Pages :
432 - 438

Corresponing Author: 
Kazem Godini
 
Authors: 
Samarghandi M.R., Arabestani M.R., Zafari D., Rahmani A.R., Afkhami A. and Godini K.
Paper ID: 
gnest_02681
Paper Status: 
Published
Date Paper Accepted: 
30/05/2018
Paper online: 
26/09/2018
Abstract: 

In this study, actual soil samples, taken from Kharg Island, Iran, with high levels of total petroleum hydrocarbons (TPHs) were bioremediated by a bacterial consortium. The strains were: Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., Brevibacillus sp. and Staphylococcus sp., which were isolated from two highly polluted sites: Kharg Island and Kermanshah Oil Refinery. The bacteria were identified and confirmed by biochemical tests and the PCR assay. In order to isolate the bacteria, the enrichment method, in which crude oil (2%) was utilized as the sole source of carbon and energy, was used. The results showed that the adapted bacterial consortium could degrade nearly 60% of the pollution. Also, it was found that after 100 days of bioremediation only Brevibacillus sp. could survive in the environment.

Keywords: 
Bioaugmentation, Pseudomonas sp., Petroleum hydrocarbons, Contaminated soil