You are hereHome | In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials
In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials View Crossref metadata(active tab) <div id="edit-xml" class="form-item form-type-item"> <?xml version="1.0"?> <doi_batch version="4.4.1" xmlns="http://www.crossref.org/schema/4.4.1" xmlns:jats="http://www.ncbi.nlm.nih.gov/JATS1" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.crossref.org/schema/4.4.1 http://www.crossref.org/schemas/crossref4.4.1.xsd"><head><doi_batch_id>000690</doi_batch_id><timestamp>1713604902</timestamp><depositor><depositor_name>University of the Aegean</depositor_name><email_address>uoa@university.com</email_address></depositor><registrant>Global NEST Journal</registrant></head><body><journal><journal_metadata><full_title>Global NEST Journal</full_title><full_title>Global NEST: the international Journal</full_title><issn media_type="print">1790-7632</issn><issn media_type="electronic">2241-777X</issn></journal_metadata><journal_issue><titles><title>Issue 3</title></titles><publication_date><month>10</month><year>2024</year></publication_date><journal_volume><volume>21</volume></journal_volume><issue>3</issue></journal_issue><journal_article><titles><title>In vitro- exploration of Fungal Endophytes of Egyptian Cynara scolymus L. (artichoke) and Investigation of some their bioactive potentials </title></titles><abstract xmlns="http://www.ncbi.nlm.nih.gov/JATS1"><p><p>Plant-associated microorganisms, especially endophytic fungi, represent an untapped resource for the discovery of biologically active natural products. The objectives of this study were to isolate, identify endophytic fungi, and produce their bioactive metabolites from the leaves of two varieties of Egyptian artichoke namely: French Hyrious and Egyptian Baladi. In addition, assess of their total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC). The results of this novel study show a total of 35 endophytic fungal species belonging to 14 genera were isolated from both artichoke leaves with gross total counts of colonizing endophytic fungi ranged from 71 to 123 cfu which is matching 78.89% to 136.67% of colonization frequency. All taxa recovered were assigned to Ascomycetes. In addition, there is high species richness and diversity indices of endophytic filamentous fungi in the leaves Baladi Artichoke as compared to its French rival. <em>Alternaria alternata</em> were found to be the most frequently isolated dominant species. The TAC, TPC and TFC of the fungal cultures ranged from 163 to 681 mgAAE/gDW, 10.38 to 40.30 mgGAE/ gDW, and 13.92 to 173.55 mgQE/gDW, respectively. Furthermore, LC-ESI-MS/MS confirmed the presence of 1,3-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid in the methanolic extract of <em>A. alternata.</em> Hence, this novel study suggested that the metabolites produced by endophytic fungi associated with Egyptian artichoke could be explored as an economic and potential natural resources with diverse pharmaceutical and biological activities.</p> </p></abstract><publication_date><month>11</month><day>21</day><year>2018</year></publication_date><pages><first_page>296</first_page><last_page>308</last_page></pages><publisher_item><identifier id_type="pii">gnest_02836</identifier></publisher_item><doi_data><doi>10.30955/gnj.002836</doi><timestamp>1713604902</timestamp><resource>https://journal.gnest.org/publication/gnest_02836</resource></doi_data></journal_article></journal></body></doi_batch> </div>
